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e coli strain c321 δa exp  (Addgene inc)


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    Addgene inc e coli strain c321 δa exp
    E Coli Strain C321 δa Exp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+c321+%CE%B4a+exp/C321%2E%CE%94A%2Eexp+(Bacterial+strain+%2349018)/pm41436063-133-0-10
    Average 93 stars, based on 63 article reviews
    e coli strain c321 δa exp - by Bioz Stars, 2026-09
    93/100 stars

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    Article Title: Comparative Analyses of the Transcriptome and Proteome of Escherichia coli C321.△A and Further Improving Its Noncanonical Amino Acids Containing Protein Expression Ability by Integration of T7 RNA Polymerase
    Article Snippet: E. coli C321.ΔA.exp , E. coli MG1655 Δ( ybhB-bioAB ):: zeoR Δ prfA ; all 321 TAG codons changed to TAA , Addgene (ID: 49018).

    Article Title: Urate oxidase-albumin conjugate, preparation method thereof, and use thereof
    Article Snippet: The method of Example 63, in which the cell line is E. coli C321.ΔA.exp(Addgene, ID:49018).

    Expressing:

    Article Title: Highly efficient and tunable system for the incorporation of unnatural amino acids into proteins in
    Article Snippet: .. E. coli C321.ΔA.exp (Addgene strain #49018) was used for expression of deGFP under the OR2-OR1-Pr promoter variants. ..

    Article Title: Multi-enzyme conjugate, method for preparing the same and method for preparing organic compound using the same
    Article Snippet: .. As an expression host for AZF-incorporated FDH (FDH-V237AZF), genomically engineered E. coli C321.ΔA.exp was obtained from Addgene (ID: 49018) and co-transformed with pEVOL-pAZF and pQE80-FDH-V237amb, affording C321.ΔA.exp [FDHV237amb], respectively. .. The mdh gene, which encodes mannitol-2-dehydrogenase originating from Pseudomonas fluorescens, with an additional C-terminal histidine sequence was synthesized by GenScript (Piscataway, N.J.) and subcloned into pQE80 to generate pQE80-MDH.

    Plasmid Preparation:

    Article Title: Albubody: an engineered scFv variant platform for site-specific drug conjugation and enhanced tumor efficacy
    Article Snippet: Antibody-drug conjugates (ADCs) represent a promising approach for targeted cancer therapy.. Although single-chain variable fragments (scFvs) hold potential as drug carriers, their application in ADCs has been limited.. This study introduces Albubody, an engineered scFv fused with an albumin-binding domain (ABD), as a potential ADC scaffold designed to interact with serum albumin in vivo.



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    e coli strain c321 δa exp  (Addgene inc)
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    E Coli Strain C321 δa Exp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+c321+%CE%B4a+exp/C321%2E%CE%94A%2Eexp+(Bacterial+strain+%2349018)/pm41436063-133-0-10
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    e coli c321δa  (Addgene inc)
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    e coli c321 δa exp strain  (Addgene inc)
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    Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the <t>E.</t> <t>coli</t> essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.
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    e coli c321 δa exp  (Addgene inc)
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    Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the <t>E.</t> <t>coli</t> essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.
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    e coli c321 δa catalog 49018 strain  (Addgene inc)
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    Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the <t>E.</t> <t>coli</t> essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.
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    amberless e coli strain c321 δa exp  (Addgene inc)
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    Addgene inc amberless e coli strain c321 δa exp
    Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the <t>E.</t> <t>coli</t> essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.
    Amberless E Coli Strain C321 δa Exp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the E. coli essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.

    Journal: Life

    Article Title: A Programmable Finite-Replicated Organism Framework for Balanced Safety and Functionality

    doi: 10.3390/life15091381

    Figure Lengend Snippet: Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the E. coli essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.

    Article Snippet: The E. coli C321.ΔA exp strain (Addgene #87359) (Lajoie MJ, 2013) was cultured in Luria–Bertani medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) supplemented with antibiotics: ampicillin (100 μg/mL), kanamycin (50 μg/mL), chloramphenicol (25 μg/mL), and spectinomycin (50 μg/mL).

    Techniques: Mutagenesis, Expressing, Construct, Two Tailed Test, Imaging, Modification